Oral Biofilm Composition, Dissemination to Keratinocytes, and Inflammatory Attenuation Depend on Probiotic and Synbiotic Strain Specificity.

Several inflammatory diseases are characterized by a disruption in the equilibrium between the host and its microbiome. Due to the increase in resistance, the use of antibiotics for the widespread, nonspecific killing of microorganisms is at risk. Pro-microbial approaches focused on stimulating or introducing beneficial species antagonistic toward pathobionts may be a viable alternative for restoring the host-microbiome equilibrium. Unfortunately, not all potential probiotic or synbiotic species and even subspecies (to strain level) are equally effective for the designated pathology, leading to conflicting accounts of their efficacy. To assess the extent of these species- and strain-specific effects, 13 probiotic candidates were evaluated for their probiotic and synbiotic potential with glycerol on in vitro oral biofilms, dissemination from biofilms to keratinocytes, and anti-inflammatory activity. Species- and strain-specific effects and efficacies were observed in how they functioned as probiotics or synbiotics by influencing oral pathobionts and commensals within biofilms and affected the dissemination of pathobionts to keratinocytes, ranging from ineffective strains to strains that reduced pathobionts by 3 + log. In addition, a minority of the candidates exhibited the ability to mitigate the inflammatory response of LPS-stimulated monocytes. For a comprehensive assessment of probiotic therapy for oral health, a judicious selection of fully characterized probiotic strains that are specifically tailored to the designated pathology is required. This approach aims to challenge the prevailing perception of probiotics, shifting the focus away from "form over function." Rather than using unproven, hypothetical probiotic strains from known genera or species, one should choose strains that are actually functional in resolving the desired pathology before labelling them probiotics.

Pediatric Odontogenic Tumors.

Odontogenic tumors are rare tumors of the jaws that arise from remnants of the tooth forming apparatus. Some odontogenic tumors demonstrate strong predilection for pediatric patients including the unicystic ameloblastoma, adenomatoid odontogenic tumor, ameloblastic fibroma, ameloblastic fibro-odontoma, odontoma, and primordial odontogenic tumor. In this review, we discuss the clinical, radiographic, histopathologic, and molecular characteristics of select odontogenic tumors that demonstrate pediatric predilection and review management.

A systematic review of medicinal plants and herbal products' effectiveness in oral health and dental cure with health promotion approach.

Medicinal plants and herbal drugs are being used increasingly as part of primary health care in most parts of the world. As important adjunctive and alternative treatments for oral health care, herbal products' use may continue to increase and become more widespread. The objective of this study is to present a comprehensive systematic review of the current published literature on the effectiveness of medicinal plants and herbal products employed to improve oral health in adolescents with a health promotion approach. The systematic review was conducted according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. The keywords "herbal medicine," "herbal extract," "herbal supplements," "plant extract," "natural drug," "pulpitis," "dental caries," "oral viral diseases," and "abscess" were used in combination with the Boolean operators OR and AND. PubMed, Embase, Scopus, and Web of Science were searched. Quality assessment of the included studies was performed using the Cochrane Handbook for Systematic Reviews of Interventions. The search yielded 49 original research studies. A total of 22 studies had low or unclear risk bias. The geographical distribution of included studies was primarily concentrated on western countries. Overall, studies reported herbal product users' age, ranging from young adults aged 18 years to elderly people aged 75 years or older. Most studies reported multiple compounds, including herbal drugs and herbal extracts. Chamomile and Aloe vera were the most frequently reported herbal compounds. The most commonly described herbal products to treat oral diseases were gels, mouth rinses, and pastes. The studies included a range of people with oral diseases, including periodontal and gingival diseases, recurrent aphthous stomatitis, oral lichen planus, and oral candidiasis. Herbal product interventions were found to be effective and safe alternatives for oral health care. One of the most important goals of the World Health Organization (until 2015) is the oral health index, so it is important that dental services be followed up more seriously. Considering the problems in reaching this goal of the World Health Organization in our country, herbal products have the ability to improve clinical oral health outcomes in adolescents. Limited adverse side effects indicate the overall safety of these treatments for a wide range of oral diseases. Therefore, the use of medicinal plants as well as alternative medicine is one of the useful methods in achieving this important goal of public health.

Relationship between stomatognathic alterations and idiopathic scoliosis: a systematic review with meta-analysis of observational studies.

Purpose: The objective of this systematic review was to assess a possible relationship between stomatognathic alterations and idiopathic scoliosis (IS). Design: This study is a systematic review with meta-analysis of observational studies. Methods: The protocol of this systematic review with meta-analysis was registered in PROSPERO (CRD42022370593). A bibliographic search was carried out in the Pubmed (MEDLINE), Scopus, Web of Science and CINAHL databases using the MeSH terms 'Scoliosis' and 'Stomatognathic Disease'. The odds ratio (OR) of prevalence and standardized mean difference (SMD) were used to synthesize the results. Results: Of 1592 studies located, 14 studies were selected with 3018 subjects (age: 13.9 years). IS was related to Angle's class II (OR = 2.052, 95% CI = 1.236-3.406) and crossbite (OR = 2.234, 95% CI = 1.639-3.045). Patients with malocclusion showed a higher prevalence of IS than controls (OR = 4.633, 95% CI = 1.467-14.628), and subjects with IS showed high overjet (SMD = 0.405, 95% CI = 0.149-0.661) and greater dysfunction due to temporomandibular disorders (SMD = 1.153, 95% CI = 0.780-1.527). Conclusion: Compared with healthy controls, subjects with IS have twice the risk of suffering from occlusion disorders, present greater temporomandibular dysfunction and have a greater overjet in the incisors. Moreover, subjects with malocclusion have an IS prevalence up to four times higher. The systematic orofacial examination of patients with IS should be recommended.

Modelo para el desarrollo de competencias profesionales específicas en la atención a pacientes con enfermedades estomatognáticas / Model for the development of specific professional competence in the care of patients with stomatognathic diseases i: en

Introducción: Las competencias profesionales son cualidades humanas que permiten una visión personalizada en un individuo, el cual se desempeña en un contexto único y, a la vez, cambiante, buscando desarrollar los recursos personológicos que debe utilizar en su vida laboral. Objetivo: Valorar la implementación de un modelo para el desarrollo de competencias profesionales específicas en la atención a pacientes con enfermedades estomatognáticas. Métodos: Se realizó un estudio en educación médica en la Facultad de Estomatología de la Universidad de Ciencias Médicas en Santiago de Cuba, durante el curso 2018-2019. La población estuvo constituida por los 59 residentes que cursaban el primer año de la especialidad de Estomatología General Integral, en quienes se aplicó un modelo para el desarrollo de competencias profesionales específicas. Durante la investigación se usaron métodos teóricos y empíricos, así como estadísticos, particularmente la distribución de frecuencias absoluta y relativa y la prueba de McNemar para determinar el nivel de desarrollo después de implementado el modelo. Resultados: Se obtuvieron opiniones positivas en el taller de socialización luego de la implementación práctica del modelo. De los residentes en estomatología, 96,4 % aprobó su pertinencia, mientras que 83,1 % estuvo muy satisfecho con él. Conclusiones: Luego de aplicado el modelo propuesto, se evidenció el desarrollo de las competencias profesionales específicas en la atención a pacientes con afecciones odontológicas.

Introduction: Professional competencies are human qualities that allow a personalized vision in an individual, who performs his functions in an unique, and at the same time, changing context, and seeks to develop the personological features that he must use in his working life. Objective: To assess the implementation of a model for the development of specific professional competence in the care of patients with stomatognathic diseases. Methods: A study in medical education was conducted at the Faculty of Dentistry in the University of Medical Sciences from Santiago de Cuba, during the 2018-2019 academic year. The population consisted of the 59 residents who were in the first year of the specialty of Comprehensive General Dentistry, in whom a model for the development of specific professional competence in dental care was applied. During the investigation theoretical and empirical methods were used, as well as statistics, particularly the distribution of absolute and relative frequencies, and McNemar test to evaluate the level of development after the model has been implemented. Results: In the socialization workshop, after the practical implementation of the model, positive opinions were obtained. Among the residents in dentistry, 96.4% approved the relevance of the model, while 83.1% were very satisfied. Conclusions: After applying the proposed model, the development of specific professional competence in the care of patients with dental conditions was evidenced.

A Review of Medical Evacuations Related to Dental Emergencies and Oral-Maxillofacial Injuries.

A literature review was performed to determine the frequency of medical evacuations (MEDEVAC) that are required for dental emergencies (DE) and oral-maxillofacial (OMF) injuries. Fourteen studies were reviewed altogether - eight which quantified evacuation of DEs or OMF injuries in military personnel (from 1982-2013) and six studies that discussed medical evacuation of DEs occurring in civilians working in offshore oil and gas rigs and wilderness expeditions (from 1976-2015). Among military personnel, DE/OMF issues were frequently among one of the top categories of medical evacuations, ranging from 2-16% of all evacuations. Among oil and gas industry workers, 5.3-14.6% of evacuations were dental-related, while one study of wilderness expeditions found that DEs ranked as the third most frequent type of injury that required evacuation. Previous studies have shown that dental and OMF problems often account for one of most frequently cited reasons for evacuation. However, due to the limited study base of DE/OMF medical evacuations, further research is needed to determine their impact on the cost of health care delivery.

Autofagia en el Contexto del Sistema Estomatognático en Enfermedades No Neoplásicas. Revisión Sistemática Exploratoria

La autofagia es un proceso de degradación lisosomal y protección celular, que está destinado a eliminar los orgánulos dañados, las proteínas mal plegadas y los patógenos intracelulares, por lo cual es un importante proceso para la salud en los humanos. La autofagia actúa como modulador de la patogénesis y es un objetivo terapéutico potencial en diversas enfermedades, como el cáncer, la diabetes o el Parkinson. En relación al sistema estomatognático, la autofagia actúa agravando o protegiendo las enfermedades orales cuando se encuentra aumentada, activada o alterada. La desregulación de los mecanismos de la autofagia repercute en el desarrollo de la autoinmunidad a través de la supervivencia de linfocitos T, participa en la disminución y degeneración de células glandulares y queratinocitos basales en patologías como el síndrome de Sjögren o el liquen plano oral; participa modulando la inflamación, pero también defendiendo a la cavidad oral del ataque de patógenos externos que pueden causar, por ejemplo, la enfermedad periodontal. Esta revisión sistemática exploratoria, describe los mecanismos generales involucrados de la autofagia en diferentes patologías no neoplásicas que afectan al sistema estomatognático.

Autophagy is a process of lysosomal degradation and cell protection, which is intended to eliminate damaged organelles, misfolded proteins, and intracellular pathogens, making it an important process for human health. Autophagy acts as a modulator of pathogenesis and is a potential therapeutic target in various diseases, such as cancer, diabetes, or Parkinson's. In relation to the stomatognathic system, autophagy acts as aggravating or protecting oral diseases when it is increased, activated, or altered. The deregulation of autophagy mechanisms affects the development of autoimmunity through the survival of T lymphocytes and participates in the decrease and degeneration of glandular cells and basal keratinocytes in pathologies such as Sjögren's syndrome or oral lichen planus; It participates by modulating inflammation, but also by defending the oral cavity from the attack of external pathogens that can cause, for example, periodontal disease. This exploratory systematic review describes the general mechanisms involved in autophagy in different non-neoplastic pathologies that affect the stomatognathic system.

Treatments for Burning Mouth Syndrome: A Network Meta-analysis.

The aim of this systematic review and network meta-analysis (NMA) of randomized controlled trials was to evaluate the effectiveness of treatments for pain relief of burning mouth syndrome (BMS). Five databases and gray literature were searched. Independent reviewers selected studies, extracted data, and assessed the risk of bias. The primary outcome was pain relief or burning sensation, and the secondary outcomes were side effects, quality of life, salivary flow, and TNF-α and interleukin 6 levels. Four comparable interventions were grouped into different network geometries to ensure the transitivity assumption for pain: photobiomodulation therapy, alpha-lipoic acid, phytotherapics, and anxiolytics/antidepressants. Mean difference (MD) and 95% CI were calculated for continuous outcomes. The minimal important difference to consider a therapy beneficial against placebo was an MD of at least -1 for relief of pain. To interpret the results, the GRADE approach for NMA was used with a minimally contextualized framework and the magnitude of the effect. Forty-four trials were included (24 in the NMA). The anxiolytic (clonazepam) probably reduces the pain of BMS when compared with placebo (MD, -1.88; 95% CI, -2.61 to -1.16; moderate certainty). Photobiomodulation therapy (MD, -1.90; 95% CI, -3.58 to -0.21) and pregabalin (MD, -2.40; 95% CI, -3.49 to -1.32) achieved the minimal important difference of a beneficial effect with low or very low certainty. Among all tested treatments, only clonazepam is likely to reduce the pain of BMS when compared with placebo. The majority of the other treatments had low and very low certainty, mainly due to imprecision, indirectness, and intransitivity. More randomized controlled trials comparing treatments against placebo are encouraged to confirm the evidence and test possible alternative treatments (PROSPERO CRD42021255039).

Evolución histórica de la prótesis bucomaxilofacial en Camagüey

Introducción: Los principios en que se sustenta el proceso revolucionario cubano y su Sistema Nacional de Salud, es tratar de dar solución a las necesidades de la población y Cuba dentro de sus limitaciones de desarrollo, ha producido cambios en las condiciones de salud, a través de su política estatal. Objetivo: Describir la evolución histórica de la prótesis bucomaxilofacial en la provincia Camagüey. Métodos: Se realizó una revisión de la de literatura, para recopilar los datos de la historia de la prótesis bucomaxilofacial. Se ejecutó una búsqueda de la literatura en las bases de datos Pubmed, Medline, Cochrane, SciELO, Hinari y prensa escrita donde se expusieran las evidencias disponibles sobre los antecedentes de prótesis bucomaxilofacial en Cuba y en la provincia Camagüey. Resultados: En Cuba la prótesis maxilofacial antes del triunfo revolucionario prácticamente no se realizaba. El 14 de junio de 1999 se crea la Red Asistencial Nacional de Rehabilitación de la Cara y Prótesis Bucomaxilofacial y en el año 2001 el Programa de Rehabilitación de la Cara y Prótesis Bucomaxilofacial, con una cobertura para todos los pacientes del país con esta necesidad de atención. En Camagüey con la creación en abril de 1975 de la consulta multidisciplinaria de Estomatología se inició la atención a los pacientes con defectos bucomaxilofacial. Esto permitió rehabilitar en esos momentos a estos enfermos con los recursos disponibles. Conclusiones: El análisis y las valoraciones realizadas determinaron que la prótesis en la provincia Camagüey ha tenido un gran desarrollo tanto asistencial como docente e investigativo, aunque no se cuenta con un servicio dedicado a la prótesis bucomaxilofacial, se han realizados labores de rehabilitación a pacientes con estos defectos, que le han permitido recuperar la estética, el estado psíquico funcional con alto grado de aceptación y satisfacción y el logro de su reincorporación a la vida social.

Introduction: The principles on which the Cuban revolutionary process and its National Health System are based, is to try to provide a solution to the needs of the population, and Cuba within its development limitations, has produced changes in health conditions, through of its state policy. Objective: To describe the historical evolution of the bucomaxillofacial prosthesis in Camagüey. Methods: A review of the literature was carried out to collect the data on the history of the bucomaxillofacial prosthesis. A search of the literature was carried out in the Pubmed, Medline, Cochrane, SciELO, Hinari and written press databases where the available evidence on the history of oral-maxillofacial prostheses in Cuba and in the Camagüey province was exposed. Results : In Cuba, before the revolutionary triumph, maxillofacial prostheses were practically not performed. On June 14, 1999, the National Assistance Network for the Rehabilitation of the Face and Bucomaxillofacial Prosthesis was created, and in 2001 the Program for the Rehabilitation of the Face and Bucomaxillofacial Prosthesis, with coverage for all patients in the country with this need for care. In Camagüey, with the creation in April 1975 of the multidisciplinary Dentistry consultation, care began for patients with oral-maxillofacial defects. This made it possible to rehabilitate these patients at that time with the resources available. Conclusions: The analysis and the evaluations carried out determined that the prosthesis in Camagüey province has had a great development in terms of care, teaching and research, although there is no service dedicated to oral and maxillofacial prostheses, rehabilitation work has been carried out on patients with these defects, which have allowed them to recover their aesthetics, their functional mental state with a high degree of acceptance and satisfaction, and the achievement of their reincorporation into social life.

Garantías explícitas en salud odontológica de las embarazadas y la salud pública por regiones y años 2010-2019 en Chile

Objetivo: Estimar la tasa de uso de las Garantías Explícitas de Salud (GES) dental de la embarazada entre 2010 a 2019 en todas las regiones de Chile. Pacientes y métodos: Diseño: descriptivo, observacional. Participantes: mujeres embarazadas, atendidas en el sistema público. Se estudió un total de 1 854 621 gestantes, 1 445 903 de gestantes con ingreso al GES y 1 257 775 de gestantes con alta dental integral pertenecientes a cada región y año analizado. Mediciones: estimación de tasas de uso del GES odontológico, análisis de las tasas de eficacia y de la evolución de las tasas de uso del GES mediante series de tiempo. Resultados: Las medias del uso del GES y la eficacia de la atención por GES fue inferior al valor óptimo (100 %). Ambas tasas difieren del valor meta de la autoridad sanitaria. Los valores de las tasas del uso del GES varían por año y región. La forma de las curvas fue predominantemente no lineal. Conclusión: El uso y la eficacia del GES odontológico en embarazadas chilenas no está en correspondencia con los objetivos del Ministerio de Salud Pública. Se requieren nuevos estudios para explicar las causas de estos resultados.

Objective. Estimate the rate of use of the Explicit Dental Health Guarantees (GES) by pregnant women from 2010 to 2019 in the regions of Chile. Patients and methods. Design: Descriptive, observational. Participants: Pregnant women, cared for in the public system. The total of pregnant women (1,854,621), pregnant women admitted to the GES (1,445,903), and pregnant women with full dental discharge (1,257,775) belonging to each of the regions and years analyzed (2010-2019) were studied. Measurements: estimation of dental GES use rates, evolution of rates over time using time series (regressions). Results. The means of the use of the GES and the effectiveness of the care by the GES was less than the optimal value (100%). Both rates differ from the target value of the health authority. The value of usage fees varies by year and region. The shape of the curves was predominantly non-linear. Conclusions. The use and effectiveness of the dental GES in Chilean pregnant women is not in correspondence with the objectives of the Ministry of Public Health. New studies are required to explain the causes of these results.

Use of Diode Laser for Lip Repositioning, Evolution of the Conventional Technique. A Case Report.

INTRODUCTION: LipStaT® lip repositioning surgery using a diode laser for the management of gummy smiles offers promising and consistent long-term results. OBJECTIVE: To describe the technique through a clinical case, including its indications, preoperative evaluation, surgical approach, and recommendations after the intervention. CASE REPORT: A 29-year-old woman with excessive gingival display (EGD) of 4 mm when smiling; the LipStaT® technique was performed with a 940 nm diode laser to remove a strip of mucosa by ablating the epithelial tissue, and thus, achieve aesthetic, harmonic and functional results. The inflammation and postoperative pain were treated with the diode laser assisted lymphatic drainage technique. After follow-up, a satisfactory tissue response was observed, and no pain or inflammation was reported. CONCLUSION: The use of a 940 nm diode laser proved to be an effective treatment option for patients with a gummy smile, allowing control of intraoperative bleeding and improvement of the healing process obtaining predictable long-term results.

De novo TRPV4 Leu619Pro variant causes a new channelopathy characterised by giant cell lesions of the jaws and skull, skeletal abnormalities and polyneuropathy.

BACKGROUND: Pathogenic germline variants in Transient Receptor Potential Vanilloid 4 Cation Channel (TRPV4) lead to channelopathies, which are phenotypically diverse and heterogeneous disorders grossly divided in neuromuscular disorders and skeletal dysplasia. We recently reported in sporadic giant cell lesions of the jaws (GCLJs) novel, somatic, heterozygous, gain-of-function mutations in TRPV4, at Met713. METHODS: Here we report two unrelated women with a de novo germline p.Leu619Pro TRPV4 variant and an overlapping systemic disorder affecting all organs individually described in TRPV4 channelopathies. RESULTS: From an early age, both patients had several lesions of the nervous system including progressive polyneuropathy, and multiple aggressive giant cell-rich lesions of the jaws and craniofacial/skull bones, and other skeletal lesions. One patient had a relatively milder disease phenotype possibly due to postzygotic somatic mosaicism. Indeed, the TRPV4 p.Leu619Pro variant was present at a lower frequency (variant allele frequency (VAF)=21.6%) than expected for a heterozygous variant as seen in the other proband, and showed variable regional frequency in the GCLJ (VAF ranging from 42% to 10%). In silico structural analysis suggests that the gain-of-function p.Leu619Pro alters the ion channel activity leading to constitutive ion leakage. CONCLUSION: Our findings define a novel polysystemic syndrome due to germline TRPV4 p.Leu619Pro and further extend the spectrum of TRPV4 channelopathies. They further highlight the convergence of TRPV4 mutations on different organ systems leading to complex phenotypes which are further mitigated by possible post-zygotic mosaicism. Treatment of this disorder is challenging, and surgical intervention of the GCLJ worsens the lesions, suggesting the future use of MEK inhibitors and TRPV4 antagonists as therapeutic modalities for unmet clinical needs.

Therapeutic effect of fluvastatin on medication-related osteonecrosis of the jaw.

BACKGROUND: Refractory jaw osteonecrosis that occurs in osteoporotic or cancer patients treated with bisphosphonates is called medication-related osteonecrosis of the jaw but its underlying mechanism is unclear. Statins, therapeutic agents for dyslipidemia, lower blood low-density lipoprotein cholesterol. Fluvastatin promotes the healing of tooth extraction sockets and reduces the risk of developing medication-related osteonecrosis of the jaw-like lesions. We used a rat model to investigate whether injecting fluvastatin at extraction sites promoted the healing of medication-related osteonecrosis of the jaw-like lesions. METHODS: Upper first molars of rats administered zoledronate and dexamethasone for 2 weeks were extracted. Two weeks after tooth extraction, rats with medication-related osteonecrosis of the jaw-like lesions (bone exposure) were included in this study. A single injection of fluvastatin was administered in the vicinity of the medication-related osteonecrosis of the jaw-like onset site in rats. RESULTS: The distance between the edges of the epithelia, the length of the necrotic bone exposed toward the oral cavity, the area of the necrotic bone, and the necrotic bone ratio were significantly smaller in the fluvastatin-administered group compared with the saline group. A single application of fluvastatin near the site of medication-related osteonecrosis of the jaw onset showed a tendency to close the epithelium, reduce necrotic bone, and form new bone, even when symptoms had already developed. CONCLUSION: This study suggests that a single topical administration of fluvastatin may be a novel treatment for medication-related osteonecrosis of the jaw.

Eficácia de diferentes tratamentos para a síndrome da ardência bucal: revisão sistemática e metanálise em rede / Efficacy of different treatments for burning mouth syndrome: systematic review and network meta-analysis

O objetivo desta revisão sistemática de ensaios clínicos randomizados (ECRs) foi avaliar a eficácia dos tratamentos para o alívio da dor da síndrome da ardência bucal (SAB). Cinco bases de dados e literatura cinzenta foram pesquisadas e as listas de referências dos estudos incluídos foram pesquisadas manualmente. Revisores independentes selecionaram estudos, extraíram dados e avaliaram o risco de viés através da ferramenta Revised Cochrane risk-of-bias tool for randomized trials (RoB 2.0). O principal desfecho foi o alívio da dor. Os desfechos secundários foram efeitos adversos, qualidade de vida, fluxo salivar, níveis de TNF-α e interleucina (IL-6), quando relatados por estudos. Para a meta análise em rede (network meta-analysis - NMA), foram agrupadas quatro intervenções comparáveis em diferentes geometrias para garantir o pressuposto da transitividade: terapia de fotobiomodulação (PBMT), ácido alfa-lipóico (ALA), fitoterápicos e ansiolíticos/antidepressivos. As estimativas de efeitos para dor foram: diferença de média (DM) para desfechos contínuos pois os estudos usaram escalas comparáveis variando de 0 a 10 para dor; e risco relativo (RR) para desfechos binários. Para qualidade de vida, a diferença de média padronizada (DMP) foi calculada pois os estudos usaram escalas diferentes. Para calcular a DM, usou-se a média e desvio padrão (DP) em baseline e no último momento de cada intervenção. Para ambas todas as estimativas, foram calculados os correspondentes intervalos de confiança (IC) de 95%. A certeza da evidência foi avaliada usando a abordagem GRADE para NMA. Para a certeza da evidência, foi avaliado ser havia problemas de risco de viés, inconsistência, evidência indireta, viés de publicação, intransitividade, imprecisão e incoerência. Para imprecisão, foi considerada a diferença mínima importante (minimal importante difference - MID) necessária para tomada de decisão de tratamento comparando intervenção e placebo, sendo este último o comparador. Para dor relatada como DM, o MID foi -1 ou 1, e 0,32 ou 1,68 para RR. A classificação de Cohen foi usada para determinar um MID de grande efeito para a qualidade de vida (DMP): < -0,8 ou >0,8. Para otimizar a interpretação dos resultados da NMA e a aplicabilidade clínica, foram usadas a abordagem GRADE minimamente contextualizada para dor e o parcialmente contextualizada para qualidade de vida. O ansiolítico (clonazepam) provavelmente reduz a dor da SAB quando comparado ao placebo (DM: - 1,88; IC 95%: -2,61; -1,16, certeza moderada). A DM do fluxo salivar aumentou ligeiramente em -0,20 tanto para o ansiolítico quanto para o placebo. A DM, para os níveis de IL-6 e TNF-α, foi maior para PBMT do que placebo, o que significa uma diminuição mais pronunciada nesses níveis para PBMT. Apesar de PBMT, pregabalina e fitoterápicos apresentarem superioridade quando comparados ao placebo, a certeza da evidência foi baixa ou muito baixa. A maioria dos demais tratamentos teve baixa e muito baixa certeza, principalmente devido à imprecisão e evidência indireta. Nenhum tratamento causou impacto na qualidade de vida. Os efeitos adversos foram pouco reportados e não influenciaram o curso dos tratamentos. Mais ECRs comparando tratamentos com placebo são encorajados para confirmar a evidência. Até o momento, o melhor tratamento para SAB é o ansiolítico clonazepam. No entanto, a aplicabilidade relacionada à eficácia, efeitos adversos e qualidade de vida são limitados à 120 dias.

This systematic review of randomized controlled trials (RCTs) aimed to assess the effectiveness of treatments for pain relief of burning mouth syndrome (BMS). Five databases and grey literature were searched, and the reference lists of included studies were hand-searched. Independent reviewers selected studies, extracted data, and assessed the risk of bias (RoB 2.0). The main outcome was pain relief. The secondary outcomes were adverse effects, quality of life, salivary flow, TNF-α and interleukin (IL-6) levels, when reported by trials. For the network meta-analysis (NMA), four comparable interventions were grouped into different geometries to ensure the transitivity assumption: photobiomodulation therapy (PBMT), alpha-lipoic acid (ALA), phytotherapics, and anxiolytics/antidepressants. The effect estimate was a mean difference (MD) for continuous outcomes instead of the standardized mean difference (SMD), as studies used comparable scales varying from 0 to 10 for pain; and risk ratio (RR) for binary outcomes. The SMD was calculated for quality of life as studies used different scales. To calculate MD, we used mean and standard deviation (SD) at the baseline and at the last time point of each intervention. For both estimates, corresponding 95% confidence intervals (CI) were calculated. The GRADE approach for NMA was used to assess the certainty of the evidence. We rated down the certainty of evidence if there were problems due to the risk of bias, inconsistency, indirectness, publication bias, intransitivity, imprecision, and incoherence. We considered the minimal important difference (MID) necessary to a treatment decision comparing intervention and placebo (comparator) to rate imprecision. For pain reported as MD, the MID was -1 or 1, and 0.32 or 1.68 for RR. The Cohen classification was used to determine a MID of large effect for the quality of life (SMD): < -0.8 or >0.8 To optimize the interpretation of results of NMA and clinical applicability, we followed the GRADE minimally contextualized framework for pain and the partially contextualized framework for quality of life. The anxiolytic (clonazepam) probably reduces pain of BMS compared to placebo (MD: - 1.88; 95% CI: -2.61; -1.16, moderate certainty). The MD of salivary flow slightly increased in -0.20 for both the anxiolytic and placebo (Heckmann et al. 2012). The MD for IL-6 and TNF-α levels was higher for PBMT than placebo, which means a more pronounced decrease in these levels for PBMT. Although PBMT, pregabalin and phytotherapics showed superiority compared to placebo, the certainty was low or very low. The majority of the other treatments had low and very low certainty, mainly due to imprecision and indirectness. No treatment improved the quality of life. Adverse effects were rarely reported and did not influence the course of treatments. More RCTs comparing treatments against placebo are encouraged to confirm the evidence. So far, the anxiolytic clonazepam is the best treatment for BMS. However, the applicability of effectiveness, adverse effects and quality of life are limited to 120 days.

Oral Health Disorders in Parkinson's Disease: More than Meets the Eye.

Despite clinical evidence of poor oral health and hygiene in Parkinson's disease (PD) patients, the mouth is often overlooked by both patients and the medical community, who generally focus on motor or psychiatric disorders considered more burdensome. Yet, oral health is in a two-way relationship with overall health-a weakened status triggering a decline in the quality of life. Here, we aim at giving a comprehensive overview of oral health disorders in PD, while identifying their etiologies and consequences. The physical (abnormal posture, muscle tone, tremor, and dyskinesia), behavioral (cognitive and neuropsychiatric disorders), and iatrogenic patterns associated with PD have an overall detrimental effect on patients' oral health, putting them at risk for other disorders (infections, aspiration, pain, malnutrition), reducing their quality of life and increasing their isolation (anxiety, depression, communication issues). Interdisciplinary cooperation for prevention, management and follow-up strategies need to be implemented at an early stage to maintain and improve patients' overall comfort and condition. Recommendations for practice, including (non-)pharmacological management strategies are discussed, with an emphasis on the neurologists' role. Of interest, the oral cavity may become a valuable tool for diagnosis and prognosis in the near future (biomarkers). This overlooked but critical issue requires further attention and interdisciplinary research.

Prevalence of Dental Caries and Dental Fluorosis among 7-12-Year-Old School Children in an Indian Subpopulation: A Cross-Sectional Study

ABSTRACT Objective: To assess the prevalence of dental caries and fluorosis among 7-12-year-old school children in Muradnagar, India. An additional objective was to determine the relationship between dental caries and fluorosis in the studied population. Material and Methods: A total of 1500 school children aged between 7 to 12 years, and both genders were randomly selected for the present study. The selected participants were divided into three groups based upon age, viz 7-8 year (group I), 9-10 year (group II) and 11-12 year (group III). Sterile mouth mirrors and explorers were used for the detection of caries. The water samples were collected to assess the fluoride concentration. The data collected were tabulated and statistically analysed using Chi-square, ANOVA, Spearman's correlation and t-test wherever applicable. Results: Out of 1500 participants, 54.1% were females and 45.9% were males. The prevalence of dental caries and fluorosis was 89.3% and 93.7%, respectively. The prevalence of caries increased with age (p<0.05) and females showed a higher prevalence in both the dentitions. Most dental fluorosis was 'very mild' (40.1%). Prevalence of dental fluorosis increased with age and males showed more fluorosis than females. A negative relationship was found between dental caries and fluorosis (p<0.05). Conclusion: Dental caries and fluorosis are the public health problems in Muradnagar; therefore, preventive programs should be organized to increase awareness among the general people.

Oral Manifestations of Wolf-Hirschhorn Syndrome: Genotype-Phenotype Correlation Analysis.

BACKGROUND: Wolf-Hirschhorn syndrome (WHS) is a rare disease caused by deletion in the distal moiety of the short arm of chromosome 4. The objectives of this study were to report the most representative oral findings of WHS, relate them with other clinical characteristics of the disease, and establish possible phenotype-genotype correlation. METHODS: The study was conducted at 6 reference centers distributed throughout Spain during 2018-2019. The study group consisted of 31 patients with WHS who underwent a standardized oral examination. Due to behavioral reasons, imaging studies were performed on only 11 of the children 6 years of age or older. All participants had previously undergone a specific medical examination for WHS, during which anatomical, functional, epilepsy-related, and genetic variables were recorded. RESULTS: The most prevalent oral manifestations were delayed tooth eruption (74.1%), bruxism (64.5%), dental agenesis (63.6%), micrognathia (60.0%), oligodontia (45.5%), and downturned corners of the mouth (32.3%). We detected strong correlation between psychomotor delay and oligodontia (p = 0.008; Cramér's V coefficient, 0.75). The size of the deletion was correlated in a statistically significant manner with the presence of oligodontia (p = 0.009; point-biserial correlation coefficient, 0.75). CONCLUSION: Certain oral manifestations prevalent in WHS can form part of the syndrome's phenotypic variability. A number of the characteristics of WHS, such as psychomotor delay and epilepsy, are correlated with oral findings such as oligodontia and bruxism. Although most genotype-phenotype correlations are currently unknown, most of them seem to be associated with larger deletions, suggesting that some oral-facial candidate genes might be outside the critical WHS region, indicating that WHS is a contiguous gene syndrome.

Recent Advances in Cone-beam CT in Oral Medicine.

BACKGROUND: The cone-beam computed tomography (CBCT) technology has continuously evolved since its appearance in oral medicine in the early 2000s. OBJECTIVES: To present recent advances in CBCT in oral medicine: i) selection of recent and consensual evidence-based sources, ii) structured summary of the information based on an iterative framework and iii) compliance with ethical, public health and patient-centered concerns. MAIN FINDINGS: We will focus on technological advances, such as sensors and reconstruction algorithms used to improve the constant quality of the image and dosimetry. CBCT examination is now performed in almost all disciplines of oral medicine: currently, the main clinical disciplines that use CBCT acquisitions are endodontics and oral surgery, with clearly defined indications. Periodontology and ear, nose and throat medicine are more recent fields of application. For a given application and indication, the smallest possible field of view must be used. One of the major challenges in contemporary healthcare is ensuring that technological developments do not take precedence over admitted standards of care. The entire volume should be reviewed in full, with a systematic approach. All findings are noted in the patient's record and explained to the patient, including incidental findings. This presupposes the person reviewing the images is sufficiently trained to interpret such images, inform the patient and organize the clinical pathway, with referrals to other medical or oral medicine specialties as needed. CONCLUSION: A close collaboration between dentists, medical physicists, radiologists, radiographers and engineers is critical for all aspects of CBCT technology.

Sialolithiasis: Application parameters for an optimal laser therapy.

In-vitro experimental parametric studies of laser ablation using natural sialoliths and artificial stones have been performed toward an efficient laser treatment of sialolithiasis. Surface microstructure and water adsorption become critical for coupling high power pulsed Ho:YAG laser radiation (λ = 2080 nm, τ ∼250 µsec), inducing ablative interactions and stone fragmentation. Results reveal a generic trend, with single pulse laser energy density threshold for sialolith ablative erosion at ∼200 J cm-2 (corresponding to intensity ∼800 kW cm-2 ) and fragmentation rates reaching ∼1 mm/pulse at ∼2400 J cm-2 . This process shows no saturation, suggesting that very high energy density irradiation at low pulse repetition rate is an efficient approach. Such operation facilitates rapid cooling and minimal thermal loading of the oral and maxillofacial area, thus causing negligible adverse effects. The method is expected to contribute to the establishment of an easy and optimal therapeutic protocol for sialolithiasis pathology.

LipStaT® Surgery, A Novel Technique for the Treatment of Gummy Smile: Case Report / LipStaT®, Una novedosa técnica quirúrgica para el tratamiento de la sonrisa gingival, reporte de casos Gummy Smile and LipStat® Technique

Background: The lip stabilization technique (LipStaT®) is a novel surgical approach for the management of gummy smile. It is an outpatient technique, minimally invasive, with low risk of morbidity and low incidence of complications. The goal of the technique is to reduce the excessive gingival display during smile. Objective: to describe the LipStaT® technique including its indications, preoperative evaluation, surgical approach and patient post procedure care instructions. Clinical Cases: the authors present two female patients, aged 20 and 31 years, with excessive gingival display (over 3mm) and with experiences of failures with other procedures. The LipStaT® technique allowed to achieve aesthetic, harmonic and functional results. Conclusion: The LipStaT® technique is a safe outpatient procedure that allows to obtain predictable results, which are well accepted by patients.

Introducción: La técnica de estabilización labial (LipStaT®), es un novedoso abordaje quirúrgico para el manejo de la sonrisa gingival. Es una técnica ambulatoria, mínima-mente invasiva, con baja morbilidad e incidencia de complicaciones. La meta de la técni-ca es reducir la excesiva cantidad de encía que se visualiza durante la sonrisa. Objetivo:Describir la técnica LipStaT® incluyendo sus indicaciones, evaluación preoperatoria, abordaje quirúrgico y las indicaciones posteriores a la intervención. Presentación del caso: Los autores presentan dos pacientes, mujeres, entre 20 y 31 años, con visuali-zación excesiva de la encía de más de 3mm al sonreír y con experiencias de fracasos con otros procedimientos. La técnica LipStaT® permitió lograr un resultado estético, armónico y funcional. Conclusión: Este es un procedimiento seguro, ambulatorio, que permite obtener resultados predecibles y bien aceptados por los pacientes.